05. How to Produce 10 Liters of Probiotic Rabal Classic (PRC)
Probiotic Rabal Classic (PRC) is a fermented product of Yeast and Lactic Acid Bacteria (RABAL) that you can produce yourself. It contains beneficial bacteria, including Lactobacillus and Yeast (a natural multivitamin).
Based on research conducted by Prof. Ibnu Sahidhir at the Brackish Water Aquaculture Development Center (BBAP) Ujung Batee Laboratory, Aceh Province—under the Ministry of Marine Affairs and Fisheries (KKP) of the Republic of Indonesia—this probiotic has been proven superior to commercially manufactured probiotics.
The Probiotic Rabal Classic (PRC) that we will produce is made from very low-cost ingredients. It is used for:
1. Improves Fish Digestive Health
Probiotic Rabal Classic (PRC) helps maintain a balanced gut microbiome in fish by suppressing the growth of pathogenic bacteria (such as Aeromonas, Vibrio, and Pseudomonas).
Enhances nutrient absorption and feed efficiency (improves FCR).
2. Boosts Fish Immune System
Probiotic Rabal Classic (PRC) stimulates the production of digestive enzymes and antibodies (e.g., IgA), while increasing lysozyme activity and phagocytosis.
Reduces the risk of infections, thereby lowering fish mortality rates.
3. Improves Pond Water Quality
Probiotic Rabal Classic (PRC) helps break down residual feed and fish waste, reducing the accumulation of toxic ammonia (NH₃) and nitrite (NO₂⁻).
Stabilizes water pH levels
Reduces unpleasant odors around the pond
4. Enhances Fish Growth
Certain Lactobacillus strains in Probiotic Rabal Classic (PRC) (such as L. plantarum and L. acidophilus) produce B-complex vitamins and organic acids that promote fish growth.
Studies demonstrate improvements in:
Daily growth rate (SGR)
Final harvest weight
5. Eco-Friendly & Safe
A natural alternative to antibiotics, reducing the risk of antibiotic resistance
Leaves no harmful residues in fish or the environment
6. Suitable Freshwater Fish Species Probiotic Rabal Classic (PRC) is effectively used for:
Catfish (Clarias sp.)
Nile tilapia (Oreochromis niloticus)
Common carp (Cyprinus carpio)
Giant gourami (Osphronemus goramy)
Pangasius catfish (Pangasius sp.)
MANUFACTURING PROCESS OF PROBIOTIC RABAL CLASSIC (PRC):
1. Ingredients:
a. Refilled/clean water = 9 liters
b. Yakult = 2 bottles
c. Tape yeast (fermentation starter) = 1 piece
d. Molasses (palm sugar syrup) = ½ liter
e. Pure coconut water (from 1 mature coconut)
f. 10-liter jerry can = 1 unit
NOTE : If you want to produce 20 liters of Probiotic Rabal Classic (PRC) in a 20-liter jerry can, simply double all the listed ingredients.
2. Processing Method for Probiotic Rabal Classic (PRC):
a. Pour 9 liters of clean water into the jerry can, then add:
2 bottles of Yakult
½ liter of molasses
1 piece of crushed tape yeast
Pure coconut water
b. Shake the jerry can for 1–2 minutes to ensure all ingredients dissolve and mix evenly.
c. If molasses is unavailable, you may substitute it with 500 grams of palm sugar.
Melt the palm sugar by heating, then let it cool before mixing it with the other ingredients in the jerry can.
d. Fermentation Process:
Store the sealed jerry can for 7 days to allow complete fermentation.
Indicators of successful fermentation:
The liquid turns brown or yellowish.
It develops a fermented/alcoholic aroma.
e. Daily Maintenance:
Open the jerry can lid daily to release fermentation gas, then reseal tightly.
Shake the jerry can for 1–2 minutes before releasing the gas to ensure proper mixing and fermentation.
Research Findings:
Ibnu Sahidhir, a Fisheries Researcher at the Brackish Water Aquaculture Development Center (BPBAP) Ujung Batee, Aceh Province, Indonesia.
Compiled by:
Arie - Aceh Fish Hatchery (Aceh Pearl Catfish Hatchery) at ATC Complex - Aceh Besar District, Indonesia.
IMPORTANT NOTICE:
The probiotic produced using this information is not authorized for commercial purposes.
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